CROP WILD RELATIVES

In order to investigate the genetic diversity and genetic status of Secale cereale L. subsp. segetale Zhuk., a weedy rye found in China, gliadin spectra of 90 S. cereale subsp. segetale populations were analyzed using the A-PAGE. The results showed a total of 34 bands were detected in all populations with eight common bands, one common band in γ-zone and seven common bands in ω-zone. The polymorphic bands taken on 76.5 %. All bands in α-, β- zone were polymorphic. The number of bands in each population ranged from 11 to 23. Each population showed unique electrophoretic pattern. The average Shannon index to four electrophoretic zones was 1.455. The highest Shannon index was found for the population 90R17, and α-, β-, γ- and ω-zone’s index detected 0.711, 1.156, 0.640 and 4.408 respectively. The lowest Shannon index was found for the population 89R55, and in α-, β-, γ- and ω-zone showed 0.000, 0.899, 0.322, and 2.709, respectively. The nearest genetic distance (0.027) was found between 90R16 and 90R35 (two Yining region’s populations), and the farthest genetic distance (0.569) was observed between 90R39 and 90R24, 90R34 (all from different regions). At Nei’s distance of 0.25, S. cereale subsp. segetale populations were clustered into five groups. AMOVA analysis indicated 14.0 % of the total genetic variation resided among populations, 86.0 % occurred within populations, and zero showed among regions. The abundant genetic diversity of implied elite genes that can be used in population diagnostics.